Journal
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
Volume 55, Issue -, Pages 15-19Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jtemb.2019.05.008
Keywords
Strontium; Rat chondrocyte; Expression; Alkaline phosphatase; Bone morphogenetic protein-4
Funding
- National Natural Science Foundation of China [31502129, 31873032, 31802154]
- Youth Innovation Team of Shaanxi Universities
Ask authors/readers for more resources
The trace element strontium has a significant impact on cartilage metabolism. However, the direct effects of strontium on alkaline phosphatase (ALP), a marker of bone growth, and bone morphogenetic protein-4 (BMP-4), which plays a key role in the regulation of bone and cartilage development, are not entirely clear. In order to understand the mechanisms involved in these processes, the chondrocytes were isolated from Wistar rat articular cartilage by enzymatic digestion and cultured under standard conditions. They were then treated with strontium at 0.5, 1.0, 2.0, 5.0, 20.0 and 100.0 mu g/mL for 72 h. The mRNA abundance and protein expression levels of ALP and BMP-4 were measured using real-time polymerase chain reaction (real-time PCR) and Western blot analysis. The results showed that the levels of expression of ALP and BMP-4 in chondrocytes increased as the concentration of strontium increased relative to the control group, and the difference became significant at 1.0 mu g/mL strontium (P < 0.05). These results indicated that strontium could be involved in cartilage development via regulating ALP and BMP-4 expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available