An enzymatic performance for a new swift magnetically detachable bio-conjugate of Candida rugosa lipase with modified Fe3O4-graphene oxide nanocomposite

The high stability, reusability, half-life, and catalytic activity, accompanied by facile separation from the reaction products, are essential for fulfilling the promise of biocatalysts for different applications in analytical and industrial processes. Here, the Candida rugosa lipase (CRL) immobilization on a new chemically modified, magnetically coated graphene oxide nanosheets is presented. The synthesis, modification, and CRL immobilization operations are monitored and verified through XRD, VSM, FT-IR, TEM, SEM, EDS, UV-Vis, and AFM techniques. The appraisal of operational parameters reveals the high-grade reusability, high pH, thermal, and storage stability of the synthesized swift magnetically detachable biocatalyst. The molecular interpretation of this catalytic performance represents by enzyme-enzyme and enzyme-product interactions. This unique magnetic bio-conjugate with outstanding performance recommends for analytical and industrial applications in a wide range of temperature and pH. Graphic abstract