Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 141, Issue 35, Pages 13734-13738Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b06092
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Funding
- Howard Hughes Medical Institute
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Targeting small-molecule fluorescent indicators using genetically encoded protein tags yields new hybrid sensors for biological imaging. Optimization of such systems requires redesign of the synthetic indicator to allow cell-specific targeting without compromising the photophysical properties or cellular performance of the small-molecule probe. We developed a bright and sensitive Ca2+ indicator by systematically exploring the relative configuration of dye and chelator, which can be targeted using the HaloTag self-labeling tag system. Our isomeric tuning approach is generalizable, yielding a farred targetable indicator to visualize Ca2+ fluxes in the primary cilium.
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