Journal
JOURNAL OF PROTEOME RESEARCH
Volume 18, Issue 11, Pages 4027-4037Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.9b00500
Keywords
target deconvolution; action mechanism; drug development; chemical biology; protein solubility; protein stability; high throughput; proteomics; mass spectrometry; tandem mass tag
Categories
Funding
- Swedish Research Council (Vetenskapsradet)
- Science for Life Laboratory
- Vetenskapsradet
- Karolinska Institutet
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Various agents, including drugs as well as nonmolecular stimuli, induce alterations in the physicochemical properties of proteins in cell lysates, living cells, and organisms. These alterations can be probed by applying a stability- and solubility-modifying factor, such as elevated temperature, to a varying degree. As a second dimension of variation, drug concentration or agent intensity/concentration can be used. Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. We envision widespread use of the PISA approach in chemical biology and drug development.
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