Journal
JOURNAL OF PROTEOME RESEARCH
Volume 18, Issue 10, Pages 3800-3806Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.9b00475
Keywords
secretome; common repository of FBS proteins; database search
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Funding
- Korea Health Industry Development Institute [HI14C3484]
- National Research Foundation [2017M3A9F9030559, 2017M3C9A5031595, 2019M3E5D3073568]
- Korea Institute of Science and Technology
- National Research Foundation of Korea [2019M3E5D3073568] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretomes. cRFP is a small supplementary sequence list of highly abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly.
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