Methods for single-cell recording and labeling in vivo

Targeting individual neurons in vivo is a key method to study the role of single cell types within local and brain-wide microcircuits. While novel technological developments now permit assessing activity from large number of cells simultaneously, there is currently no better solution than glass micropipettes to relate the physiology and morphology of single-cells. Sharp intracellular, juxtacellular, loose-patch and whole-cell approaches are some of the configurations used to record and label individual neurons. Here, we review procedures to establish successful electrophysiological recordings in vivo followed by appropriate labeling for post hoc morphological analysis. We provide operational recommendations for optimizing each configuration and a generic framework for functional, neurochemical and morphological identification of the different cell-types in a given region. Finally, we highlight emerging approaches that are challenging our current paradigms for single-cell recording and labeling in the living brain.