Journal
JOURNAL OF NATURAL PRODUCTS
Volume 82, Issue 8, Pages 2189-2200Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jnatprod.9b00205
Keywords
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Funding
- Science and Technology Plan Project of Guangdong Province [2017A050501022]
- Open Project Program of Provincial Key Laboratory of Green Processing Technology and Product Safety of Natural Products [ICL-2018-02]
- Innovation Teams of Modern Agricultural Industry Technology System in Guangdong Province [2016LM1087, 2016LM2151]
- Key Research and Development Program of Guangdong Province [2019B020213001]
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1-Deoxynojirimycin (DNJ) exerts hypoglycemic effects. However, the traditional method for DNJ extraction is inefficient, and the hypoglycemic mechanism of DNJ remains unclear. In this study, the mixed fermentation by Lactobacillus fermentum and Saccharomyces cerevisiae was used to enhance DNJ extraction efficiency. It was found that this strategy was more efficient than the traditional method as the yield improved from the original 3.24 mg/g to 5.97 mg/g. The purified DNJ significantly decreased serum glucose (P < 0.01) and insulin levels (P < 0.05), improved serum lipid levels (P < 0.05), and reversed insulin resistance (P < 0.05) in diabetic mice. These changes were caused by up-regulating the protein expression of insulin receptor and glycolysis enzymes (GK, PK, and PFK) (P < 0.05) and down regulating the protein expression of insulin receptor substrate-1 and gluconeogenesis enzymes (PCB, PEPCK, FBPase, and G-6-Pase) (P < 0.0.5), thus alleviating glucose tolerance. Additionally, DNJ treatment relieved gut dysbiosis in diabetic mice by promoting the growth of Lactobacillus, Lachnospiraceae NK4A136 group, Oscillibacter, norank Lachnospiraceae, Alistipes, and Bifidobacterium (P < 0.05) and suppressing the growth of Ruminococcaceae UCG-014, Weissella, Ruminococcus, Prevotellaceae Ga6A1 group, Anaerostipes, Klebsiella, Prevotellaceae UCG-001, and Bacteroidales S24-7 group (P < 0.05).
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