Journal
JOURNAL OF GENERAL VIROLOGY
Volume 100, Issue 10, Pages 1442-1456Publisher
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.001290
Keywords
ASPV; CP gene; deletions; phylogenetic analysis; population genetics; recombination
Categories
Funding
- National Science Centre of Poland [2011/03/B/NZ9/01680]
- Ministerio de Ciencia, Innovacion y Universidades - FEDER [BFU2015-65037-P]
Ask authors/readers for more resources
In this study, 267 coat protein gene (CP) sequences from 48 Polish isolates of Apple stem pitting virus (ASPV) were determined. The genetic structure of the virus population was analysed and possible mechanisms of molecular evolution explored. We found evidence of recombination within the ASPV population and the presence of 17 ASPV molecular variants that differ in the length, number and arrangement of deletions in the CP. Population genetic analyses showed significant variation among isolates from pear and apple trees, between isolates from the same host species and, more interestingly, within isolates, supporting the existence of significant levels of variability within individual hosts, as expected by a quasispecies population structure. In addition, different tests support that selection might have been an important force driving diversification within isolates: positive selection was found acting upon certain amino acids. Phylogenetic analyses also showed that isolates did not classify according to the host species (pear or apple trees) but according to the pattern of deletions, suggesting a possible role for deletions during clade diversification.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available