Journal
JOURNAL OF FOOD PROTECTION
Volume 82, Issue 8, Pages 1292-1299Publisher
INT ASSOC FOOD PROTECTION
DOI: 10.4315/0362-028X.JFP-18-581
Keywords
Adsorption; Deoxynivalenol; Detoxification; Kinetics; Lactobacillus paracasei
Funding
- 12th Five-Year'' National Science and Technology Support program [2015BAD16B04]
Ask authors/readers for more resources
Deoxynivalenol (DON) is a potent mycotoxin produced by many Fusarium spp. that invade grains during the growth and storage seasons. Lactic acid bacteria have been reported to be capable of removing several toxins, thereby providing an effective detoxification method for possible contaminated substrates. The present study mainly focused on investigating the detoxification characteristics of DON by a Lactobacillus paracasei LHZ-1 strain, which was recently isolated from yogurt with a strong promise of removing DON from liquid culture. The results obtained showed that the cell wall of L. paracasei LHZ-1 can remove up to 40.7% of 50 mu g/mL DON, whereas only 10.5 and 8.9% are removed by the culture supernatant or cellular lysate, respectively. Laser scanning confocal microscopy helped to identify the mechanism of DON detoxification by L. paracasei LHZ-1 through cellular adsorption, where DON was found to bind to the surface of bacterial cells to form complexes. In stability tests, about 39 or 99% of bound DON, either to viable bacterial cells or heat-inactivated cells, respectively, was released by methanol extractions, which indicated that the binding force between viable cells and DON could be stronger than it is in heat-inactivated cells. Adsorption kinetics demonstrated that approximately 33% of DON was removed within 20 h, with a maximum adsorption capacity of approximately 50.5 mu g/mL in phosphate-buffered solution.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available