Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 847, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2019.113232
Keywords
Flavocytochrome c(3); Shewanella putrefaciens; pH-dependent redox activity; Fumarate reductase; Catechol pH probe; Cyclic voltammetry; Electrode surface modification
Categories
Funding
- Marie Sklodowska Curie Individual Fellowship through the European Union's Horizon 2020 research and innovation programme [745689]
- Fundacao para a Ciencia e a Tecnologia [SFRH/BPD/93164/2013]
- Universidade Nova de Lisboa from the European Union's Horizon 2020 WIDESPREAD - Twinning research and innovation programme [810856]
- Fundacao para a Ciencia e a Tecnologia (FCT) Portugal [PTDC/BBB-BQB/4178/2014, PTDC/BIA-BQM/30176/2017]
- FEDER funds through COMPETE2020 - Programa Operacional Competitividade e Internacionalizageo (POO) [LISBOA-01-0145-FEDER-007660]
- France-Portugal PHC PESSOA programme [408142E]
- Marie Curie Actions (MSCA) [745689] Funding Source: Marie Curie Actions (MSCA)
- Fundação para a Ciência e a Tecnologia [PTDC/BBB-BQB/4178/2014, PTDC/BIA-BQM/30176/2017] Funding Source: FCT
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The electroactivity of adsorbed flavocytochrome c(3), a tetraheme FAD-containing flavoenzyme isolated from the bacterium Shewanella putrefaciens, is investigated by cyclic voltammetry at an edge plane pyrolytic graphite electrode before and after modification with grafted catechol serving as an efficient pH sensor based on a redox readout. Flavocytochrome c(3) adsorption onto the unmodified or modified electrode surface is successfully achieved by cyclic voltammetry (100 consecutive cycles) in a flavocytochrome c(3) solution containing polymyxin as co-adsorbate. The immobilized flavocytochrome c(3) retains its electrochemical activity and its catalytic fumarate reductase activity. The redox activity of the protein arises from its FAD and four hemes cofactors. The experiments evidence that the hemes' redox potential of flavocytochrome c(3) from Shewanella putrefaciens, for which no crystal structure is yet available, depend on pH which is at variance with data from the other strains Shewanella frigidimarina or Shewanella oneidensis.
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