Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1125, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2019.121715
Keywords
MOF-polymer monolithic column; On-line solid phase extraction; UA; HPLC
Funding
- National Natural Science Foundation of China [21575033, 21505030]
- Natural Science Foundation of Hebei Province [B2018201270]
Ask authors/readers for more resources
A metal organic framework (MOF)-polymer monolithic column was prepared by redox initiation using modified MOF and N-methylolacrylamide (NMA) as co-monomers. The obtained monolithic column was characterized by scanning electron microscopy (SEM) and nitrogen adsorption-desorption isotherm measurement. It was used as a solid phase extraction (SPE) absorbent for the online enrichment of ursolic acid (UA) by high performance liquid chromatography. The adsorption amount of UA on the monolith was compared with that of silica gel-C18 adsorbent and the monolith without MOF material. The MOF-polymer monolithic column showed high selectivity and good permeability. Under the optimum conditions for extraction and determination, the calibration equation was y = 79.854 x + 0.1939; the linear range was 0.001-0.9 mg/mL; the linear regression coefficient was 0.9993; the limit of detection (LOD) and the limit of quantification (LOQ) were 0.17 mu g/mL and 0.57 mu g/mL, respectively; the inter-day and intra-day accuracies were < 6.44%; the recovery was in the range of 86.52-105.26%. The MOF-polymer monolithic column was successfully used as SPE column for enrichment and determination of UA in Chinese herbal medicine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available