Fluorinated diiodine alkanes exert developmental toxicity on embryo-larval stages of zebrafish strain AB via regulating the expression of the specific endocrine-related genes

Fluorinated diiodine alkanes (FDIAs) are environmental pollutants, including octafluoro-1,4-diiodobutane (PFBDI), hexadecafluoro-1,8-diiodooctane (PFODI) and dodecafluoro-1,6-diiodohexane (PFHxDI). They showed an estrogenic effect in in vitro studies. However, little information is currently available regarding the toxicity of FDIAs in in vivo studies. Zebrafish (Danio rerio) is a vertebrate animal model that is increasingly used for toxicity and efficacy screening as well as for assessing the toxicity and safety of novel compounds, pollutants and pharmaceuticals. In the present study, we investigated the developmental toxicity of FDIAs (PFBDI, PFHxDI and PFODI) and the specific endocrine-related gene expression in zebrafish embryos. The results revealed that all three FDIAs showed developmental toxicity on zebrafish embryos. The half-maximal effective concentration values for PFBDI, PFHxDI and PFODI were 0.89 +/- 0.07, 0.53 +/- 0.04 and 0.04 +/- 0.007 mm, respectively. PFHxDI exhibited the highest developmental toxicity compared with the other FDIAs. In addition, all three FDIAs significantly upregulated the expression of estrogen receptor (esr)1 and cytochrome P450 (CYP) 19b (CYP19b), but did not significantly affect the expression of esr2b, CYP17 and CYP19a in zebrafish. The upregulation effect of PFHxDI was greater than the effect of PFBDI and PFODI. This study furthers our knowledge on the effects of FDIAs on the developmental toxicity and the specific endocrine-related gene expression in the embryo-larval stages of zebrafish. Our results provided a preliminary insight into the toxicity of FDIAs in zebrafish, which will be of great relevance regarding future studies on FDIAs in the environment.