Journal
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
Volume 145, Issue 2, Pages 666-+Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2019.08.006
Keywords
Severe asthma; type 2-low asthma; IL-10; immune regulation; T cell; macrophage; dendritic cell; IFN-gamma
Categories
Funding
- Wellcome Trust Senior Fellowship [107059/Z/15/Z]
- Medical Research Council
- Francis Crick Institute from Cancer Research UK [FC001126]
- UK Medical Research Council [FC001126]
- Wellcome Trust [FC001126]
- Asthma UK PhD studentship
- MRC [MC_U117565642] Funding Source: UKRI
Ask authors/readers for more resources
Background: Although originally defined as a type 2 (T2) immune-mediated condition, non-T2 cytokines, such as IFN-gamma and IL-17A, have been implicated in asthma pathogenesis, particularly in patients with severe disease. IL-10 regulates T-H cell phenotypes and can dampen T2 immunity to allergens, but its functions in controlling non-T2 cytokine responses in asthmatic patients are unclear. Objective: We sought to determine how IL-10 regulates the balance of T-H cell responses to inhaled allergen. Methods: Allergic airway disease was induced in wild-type, IL-10 reporter, and conditional IL-10 or IL-10 receptor a (IL-10Ra) knockout mice by means of repeated intranasal administration of house dust mite (HDM). IL-10 and IFN-gamma signaling were disrupted by using blocking antibodies. Results: Repeated HDM inhalation induced a mixed IL-13/IL-17A response and accumulation of IL-10-producing forkhead box P3-negative effector CD4(+) T cells in the lungs. Ablation of T cell-derived IL-10 increased the IFN-gamma and IL-17A response to HDM, reducing IL-13 levels and airway eosinophilia without affecting IgE levels or airway hyperresponsiveness. The increased IFN-gamma response could be recapitulated by IL-10R alpha deletion in CD11c(+) myeloid cells or local IL-10R alpha blockade. Disruption of the T cell-myeloid IL-10 axis resulted in increased pulmonary monocyte-derived dendritic cell numbers and increased IFN-gamma-dependent expression of CXCR3 ligands by airway macrophages, which is suggestive of a feedforward loop of TH1 cell recruitment. Augmented IFN-gamma responses in the HDM allergic airway disease model were accompanied by increased disruption of airway epithelium, which was reversed by therapeutic blockade of IFN-gamma. Conclusions: IL-10 from effector T cells signals to CD11c(+) myeloid cells to suppress an atypical and pathogenic IFN-gamma response to inhaled HDM.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available