Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 67, Issue 32, Pages 8905-8918Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b02331
Keywords
NAC transcriptional repressor; maize; osmotic stress; ABA; reactive oxygen species; proline biosynthesis
Funding
- National Natural Science Foundation of China [31701328]
- Postdoctoral Science Foundation of Heilongjiang Province [LBH-Z16165]
- Program for Young Scholars with Creative Talents in Heilongjiang Bayi Agricultural University [YJSCX-2017Y01]
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NAC TFs play crucial roles in response to abiotic stresses in plants. Here, ZmNAC071 was identified as a nuclear located transcriptional repressor. Overexpression of ZmNAC071 in Arabidopsis enhanced sensitivity of transgenic plants to ABA and osmotic stress. The expression levels of SODs, PODs, P5CSs, and AtMYB61 were inhibited by ZmNAC071, which results in reduced ROS scavenging and proline content, increased ROS level, and water loss. Besides, the expression levels of some ABA or abiotic stress-related genes, like ABIs, RD29A, DREBs, and LEAs were also significantly inhibited by ZmNAC071. Yeast one-hybrid assay demonstrated that ZmNAC071 specifically bound to the cis-acting elements containing CGT[G/A] core sequences in the promoter of stress-related genes, suggesting that ZmNAC071 may participate in the regulation of transcription of these genes through recognizing the core sequences CGT[G/A]. These results will facilitate further studies concerning the cis-elements and downstream genes targeted by ZmNAC071 in maize.
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