Inhibitory activities of biflavonoids against amyloid-β peptide 42 cytotoxicity in PC-12 cells

A major hallmark of Alzheimer's disease is the cerebral accumulation and resulting cytotoxicity of amyloid-beta peptides, particularly A beta 42. In this study, we used an MTT assay to investigate the inhibitory activity of biflavonoids 1-22 against A beta 42 cytotoxicity in PC-12 cell cultures. Cytoprotective effects were observed for the following amentoflavone type biflavonoids: podocarpusflavone B 8, isoginkgetin 10, sciadopitysin 13, and kayaflavone 15. These biflavonoids exhibited strong activity in tested compounds, with EC50 values of 5.18, 10.77, 9.84, and 5.29 mu M, respectively. Cell viability tests of PC-12 cells revealed that biflavonoids 13 and 15 had stronger inhibitory activities than apigenin 23 and (-)-epigallocatechin gallate 24. (C) 2015 Elsevier Ltd. All rights reserved.