Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 142, Issue -, Pages 172-180Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.09.089
Keywords
Aflatoxin B-1; Bunium persicum essential oil; Chitosan nanopolymer
Funding
- Science and Engineering Research Board New Delhi, India [ECR/2016/000299]
- UGC [370673]
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The present study reports the antifungal, aflatoxin B-1 inhibitory, and free radical scavenging activity of chitosan-based nanoencapsulated Bunium persicum Boiss. essential oil (Ne-BPEO). The chemical profile of BPEO was identified through Gas chromatography mass spectrometry analysis where cuminaldehyde (21.23%), sabinene (14.66%), and gamma-terpinen (12.49%) were identified as the major compounds. Ne-BPEO was prepared using chitosan and characterised by Scanning electron microscope (SEM), Atomic force microscope (AFM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) assay. Ne-BPEO completely inhibited the growth and aflatoxin B-1 production at a concentration of 0.3 mu L/mL. The antifungal and aflatoxin B-1 inhibitory effects were related to decreasing in ergosterol content, leakage of membrane ions (Ca2+, K+, and Mg2+), impairment in carbohydrate catabolism, and functioning of ver-1 gene of A. flavus exposed to Ne-BPEO over the control. In addition, Ne-BPEO exhibited promising free radical scavenging activity through DPPH assay (IC50 12.64 mu L/mL) with high thermo-stability. Therefore, chitosan could be used as a carrier agent of plant-based preservative to enhance the shelf-life of food products against A. flavus and aflatoxin B-1 contamination. (C) 2019 Elsevier B.V. All rights reserved.
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