Journal
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
Volume 113, Issue -, Pages 87-94Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biocel.2019.06.004
Keywords
bFGF; ASC; HPAEC; PI3k/Akt; PAH
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Funding
- Youth Subject of Jiangsu Provincial Commission of Health and Family Planning [Q2017009]
- Postgraduate Student Research and Practise Innovation Project of Jiangsu Province [KYCX17-1935]
- Key Technology Research of Nantong People's Livelihood [MS32015025]
- National Natural Science Foundation of China [81503143]
- Six Talent Peaks Project in Jiangsu Province [2015- WSW-049, 2015-WSN-062]
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Pulmonary arterial hypertension (PAH) is characterized as pulmonary arterial endothelial dysfunction and endothelial cells over proliferation, therefore, the repair of pulmonary arterial endothelial cells has been a common goal in treating PAH. In the present study, human adipose derived mesenchymal stem cells (ASCs) were transfected with bFGF lentiviral vector and co-cultured with monocrotaline pyrrole treated human pulmonary arterial endothelial cells (HPAECs). The results showed that bFGF-ASCs improved the proliferation, viability and decreased the apoptosis of HPAECs, besides, improved PAH was observed in PAH rat models. Western blot analysis showed that the PI3k and p-Akt protein expression level increased in HPAECs, suggesting the activation of the PI3k/Akt signaling pathway. With the administration of LY294002, the bFGF induced HPAECs survival and PI3k/Akt signaling activation were successfully blocked. The present study demonstrated that bFGF transfected ASCs improved the survival of HPAECs by activating the PI3k/Akt pathway.
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