Journal
GENESIS
Volume 58, Issue 1, Pages -Publisher
WILEY
DOI: 10.1002/dvg.23340
Keywords
CRISPR; Cas9; genome editing; knock-in; nefma; zebrafish
Categories
Funding
- Universite Pierre et Marie Curie
- Centre National de la Recherche Scientifique
Ask authors/readers for more resources
CRISPR/Cas9-based strategies are widely used for genome editing in many organisms, including zebrafish. Although most applications consist in introducing double strand break (DSB)-induced mutations, it is also possible to use CRISPR/Cas9 to enhance homology directed repair (HDR) at a chosen genomic location to create knock-ins with optimally controlled precision. Here, we describe the use of CRISPR/Cas9-targeted DSB followed by HDR to generate zebrafish transgenic lines where exogenous coding sequences are added in the nefma gene, in frame with the endogenous coding sequence. The resulting knock-in embryos express the added gene (fluorescent reporter or KalTA4 transactivator) specifically in the populations of neurons that express nefma, making them convenient tools for research on these populations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available