Journal
GENE
Volume 710, Issue -, Pages 258-264Publisher
ELSEVIER
DOI: 10.1016/j.gene.2019.06.004
Keywords
Breast cancer; Exosomes; LncRNAs; Pulmonary metastasis; Microenvironment
Categories
Funding
- National Natural Science Foundation of China [31601156]
- Jiangsu Science and Technology Association Young Science and Technology Talents Enrollment Project
- Changzhou Sci Tech Program [CJ20180062]
- Project of Jiangsu Provincial Medical Youth Talent [QNRC2016267]
- Changzhou Applied Basic Research Guiding Project [2017309]
- Jiangsu Province 333 Talent Project [BRA2018170]
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Objective: Increasing evidence indicated that cancer-secreted exosomes played an important role in tumor metastasis. However, the function of exosomes in breast cancer pulmonary metastasis remains unknown. The aim of the study was to investigate the role of exosome-derived from breast cancer-secreted long non-coding RNAs (LncRNAs) on pre-metastatic niche formation in pulmonary metastasis. Methods: Exosomes-derived from breast cancer were separated by ultracentrifugation. The high-throughput sequencing, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were used to detect and evaluate the differential expression of LncRNAs in lung fibroblasts with exosomes treated. And quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify candidate LncRNAs expression. Results: We found that exosomes-derived from breast cancer induced lung fibroblasts proliferation and migration. In addition, a large number of LncRNAs expression abnormalities were involved in the breast cancer lung metastasis microenvironment. Conclusion: Our findings suggested that exosomal LncRNAs facilitated tumor pre-metastatic niche formation and represented a novel mechanistic insight into the molecular mechanism of cancer metastasis microenvironment.
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