Journal
FOOD HYDROCOLLOIDS
Volume 93, Issue -, Pages 293-307Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2019.02.040
Keywords
Nanotubes; Layer-by-layer; ITC; AFM; Cell viability; HeLa cells
Categories
Funding
- William R. Scholle endowment
Ask authors/readers for more resources
An exciting technology which still has many unknowns is the fabrication of hollow cylindrical polyelectrolyte complexes that can function at different pHs. With two-open ends and hollow interior, bionanotubes can find application in nanodevices, pharmaceutics, and biology. This study focused on fabricating nanotubes using chitosan (CHI) with alpha-lactalbumin (LAC), and bovine serum albumine (BSA) with kappa-carrageenan (CAR). The zeta-potential charge difference was largest and therefore optimum at pH 7.0 for CHI/LAC and pH 4.0 for BSA/CAR. Strong electrostatic interactions enable the formation of bilayers from oppositely charged polyelectrolytes. ITC was able to show differences in the strength and spontaneity of electrostatic bonding interactions between both pairs of polyelectrolytes at these pHs. Nanotubes with 400, 600 and 800 nm diameter were achieved with a total of 5 bilayers for the 600 and 800 nm diameter nanotubes and 4 bilayers with the 400 nm diameter nanotubes using polycarbonate membranes. SEM images showed the formation of well-defined nanotubular structures that were affected by the type of polyelectrolytes used. The mechanical strength of the walls of the nanotubes was dependent on the polyelectrolytes used and the diameter of each nanotube, as shown by atomic force microscopy (AFM). Young's moduli, in the range of 25-55 MPa were obtained for LAC/CHI, and were significantly different from BSA/CAR nanotubes with values around 30-80 MPa. Both nanotubes systems proved to be potential candidates for the encapsulation and delivery of curcumin. These nanotubes achieved entrapment efficiencies around 40-45% with subsequent releasing in physiological conditions up to 300 mu g/ml, with no significant cytotoxicity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available