L-Glutamate treatment enhances disease resistance of tomato fruit by inducing the expression of glutamate receptors and the accumulation of amino acids

Gray mold caused by Botrytis cinerea is the most important disease in postharvest tomato fruit. Inducing resistance to fungal pathogens in the harvested fruit and vegetable is a promising approach to control postharvest losses. In the present study, the effect of L-glutamate on induction of resistance to B. cinerea and the underlying mechanisms were investigated. The results indicated that L-glutamate at 100 ppm was effective in reducing the gray mold of tomatoes after inoculation of the pathogen. Gene expressions of nine glutamate receptors, four pathogenesis-related proteins and the content of amino acids were affected by L-glutamate treatment. Furthermore, the metabolites of L-glutamate, including GABA, Met, Lys and Arg, could also induce significant resistance against B. cinerea in tomato fruit. Our findings suggested that L-glutamate treatment may represent a promising method for managing postharvest decay of tomato fruit.