Journal
FOOD CHEMISTRY
Volume 288, Issue -, Pages 102-107Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.02.024
Keywords
Fructose syrup; beta-Galactosidase; Glucose isomerase; Cascade reaction; combi-CLEAs
Funding
- Chilean Fondecyt [1160768]
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A new bi-enzymatic catalyst has been produced by precipitation and crosslinking (combi-CLEAs) of beta-galacto-sidase and glucose isomerase for catalyzing the cascade reactions of lactose conversion into fructose, producing a lactose-fructose syrup (LFS). Glucose isomerase was chemically aminated to increase its reactive surface groups for favour the crosslinking step. The effect of beta-galactosidase to glucose isomerase activity ratio and glutaraldehyde to protein mass ratio in combi-CLEAs production was evaluated. The selected combi-catalyst was successfully used in the production of fructose syrup from lactose in a single reaction vessel. The biocatalyst could be used at least in five sequential batches of LFS production, remaining fully stable after a total of 50 h of reaction, obtaining a product of constant quality. A robust bi-enzymatic catalyst was produced that can be repeatedly used in LFS production, an attractive mild sweetener for the dairy food industry.
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