Journal
FOOD CHEMISTRY
Volume 293, Issue -, Pages 438-445Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.05.003
Keywords
Deep eutectic solvents; Common buckwheat sprouts; Flavonoids; Optimum extraction; Response surface methodology
Funding
- Main Research Program of the Korea Food Research Institute - Ministry of Science and ICT [E0187200-02]
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In this study, deep eutectic solvents (DESs) coupled with ultrasound-assisted extraction (UAE) were evaluated and optimized for the extraction of major flavonoids from common buckwheat sprouts. Initially, 18 different choline chloride (CC)-based DESs were evaluated as potential extraction solvents, and 80% CCTG (CC-based DES composed of triethylene glycol and 20 vol% water) extracted significantly higher (p < 0.05) amounts of flavonoids than other DESs studied. The extraction efficiency of 80% CCTG was even higher (p < 0.05) than that of methanol for the extraction of vitexin and quercetin-3-O-robinobioside. Procedure using 80% CCTG and UAE were optimized; an extraction temperature of 56 degrees C and extraction time of 40 min were considered optimal. The optimized extraction procedure was reliable and efficient for the extraction of major flavonoids from common buckwheat sprouts. In addition, flavonoids could be efficiently recovered from DES extracts with high recovery yields (> 97%) by using a C18 solid-phase extraction.
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