Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 92, Issue -, Pages 782-791Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2019.07.010
Keywords
Toll-like receptor; Spotted sea bass; Bacterial infection; Innate immunity; Genome; Expression
Funding
- National Key R&D Program of China [2018YFD0900101]
- National Natural Science Foundation of China [31602147]
- China Agricultural Research System [CARS-47]
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Toll-like receptor (TLR) genes are the earliest reported pathogen recognition receptors (PRRs) and have been extensively studied. These genes play pivotal roles in the innate immune defense against pathogen invasion. In this study, a total of 16 tlr genes were identified and characterized in spotted sea bass (Lateolabrax maculatus). The tlr genes of spotted sea bass were classified into five subfamilies (tlr1-subfamily, tlr3-subfamily, tlr5-subfamily, tlr7-subfamily, and tlr1-subfamily) according to the phylogenetic analysis, and their annotations were confirmed by a syntenic analysis. The protein domain analysis indicated that most dr genes had the following three major TLR protein domains: a leucine-rich repeat (LEER) domain, a transmembrane region (TM) and a Toll/interleukin-1 receptor (TER) domain. The tlr genes in spotted sea bass were distributed in 11 of 24 chromosomes. The mRNA expression levels of 16 dr genes in response to Vibrio harveyi infection were quantified in the head kidney. Most genes were downregulated following V. harveyi infection, while only 5 tlr genes, including tlr1-1, tlr2-2, tlr5, and tlr7, were significantly upregulated. Collectively, these results help elucidate the crucial roles of dr genes in the immune response of spotted sea bass and may supply valuable genomic resources for future studies investigating fish disease management.
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