Journal
DEVELOPMENTAL DYNAMICS
Volume 248, Issue 10, Pages 1009-1019Publisher
WILEY
DOI: 10.1002/dvdy.97
Keywords
frontal bone; mesoderm; microRNA; neural crest; parietal bone; skull vault
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Funding
- Department of Sci-Tech of Zhejiang Province [LGF19H140002]
- National Natural Science Foundation of China [81400489]
- Sci-Tech Planning Project of Zhejiang Province [2014C33122, 229388]
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Background The murine calvaria has several membrane bones with different tissue origins (e.g., neural crest-derived frontal bone vs. mesoderm-derived parietal bone). Neural crest-derived frontal bone exhibits superior osteogenic activities and bone regeneration. MicroRNA (miRNA) has been emerged as a crucial regulator during organogenesis and is involved in a range of developmental processes. However, the underlying roles of miRNA regulation in frontal bone and parietal bone is unknown. Results Total of 83 significantly expressed known miRNAs were identified in frontal bones versus parietal bones. The significantly enriched gene ontology and KEGG pathway that were predicted by the enrichment miRNAs were involved in several biological processes (cell differentiation, cell adhesion, and transcription), and multiple osteogenic pathways (e.g., focal adhesion, MAPK, VEGF, Wnt, and insulin signaling pathway. Focal adhesion and insulin signaling pathway were selected for target verification and functional analysis, and several genes were predicted to be targets genes by the differentially expressed miRNAs, and these targets genes were tested with significant expressions. Conclusions Our results revealed a novel pattern of miRNAs in murine calvaria with dual tissue origins, and explorations of these miRNAs will be valuable for the translational studies to enhance osteogenic potential and bone regeneration in the clinic.
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