Duplex-forming Oligonucleotide of Triazole-linked RNA

An oligonucleotide of triazole-linked RNA ((TL)RNA) was synthesized by performing consecutive copper-catalyzed azide-alkyne cycloaddition reactions for elongation. The reaction conditions that had been optimized for the synthesis of 3-mer (TL)RNA were found to be inappropriate for longer oligonucleotides, and the conditions were reoptimized for the solid-phase synthesis of an 11-mer (TL)RNA oligonucleotide. Duplex formation of the 11-mer (TL)RNA oligonucleotide was examined with the complementary oligonucleotide of natural RNA to reveal the effects of the 2 '-OH groups on the duplex stability.