Journal
CARBOHYDRATE POLYMERS
Volume 218, Issue -, Pages 37-45Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.04.057
Keywords
Schizophyllum commune; Polysaccharides; Immunomodulatory; RAW264.7 cell
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) [2016R1A2B4011743, 2018R1A6A1A03023584]
- National Research Foundation of Korea [2016R1A2B4011743, 2018R1A6A1A03023584] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Polysaccharides were extracted from Schizophyllum commune (a common mushroom) and their structural and immune-enhancing properties were investigated. Crude and fractions (F-1 and F-2) were composed of sugars (50.3-82.8%), proteins (1.46-20.1%), and sulfates (1.33-7.01%). Monosaccharide compositions of Cr and F-1 were mainly composed of glucose (75.5% and 88.2%) with small amounts of mannose, galactose and xylose whereas the F-2 was mainly composed of manose (55.2%) with minor amounts of galactose, glucose, and xylose. Their immune-enhancing activities were tested using RAW264.7 cells. Proliferation activity of RAW264.7 cells was over 100% after treatment with these polysaccharides. In addition, RAW264.7 cells produced large amounts of nitric oxide and various cytokines by up-regulating mRNA expression levels and the activation of nuclear factor-kappa (NF-kappa B) and mitogen-activated protein kinase (MAPK) after treatment with these polysaccharides. In addition, RAW264.7 cells were activated mainly through CR-3 and TLR-4 receptors. The backbone of F-2 with excellent immune-enhancing activity was mainly linked by (1 -> 3)-linked-mannopyranosyl and (1 -> 2,3)-linked-mannopyranosyl residues.
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