Journal
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Volume 66, Issue 5, Pages 720-730Publisher
WILEY
DOI: 10.1002/bab.1804
Keywords
cellulosome; biorefinery; degradation; reconstitution; surface display
Funding
- National Natural Science Foundation of China [51878291] Funding Source: Medline
- Yunnan Province Major Projects of Improving Flue-curing Quality and Efficiency Funding Source: Medline
- State Key Laboratory of Pulp and Paper Engineering [2017TS06] Funding Source: Medline
- Yunnan Applied Basic Research Project [2018BB019] Funding Source: Medline
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Lignocellulose, one of the most abundant renewable sources of sugar, can be converted into bioenergy through hydrolysis of cellulose and hemicellulose. Due to its renewability and availability in large quantities, bioenergy is considered as a possible alternative to fossil energy and attracts the attention of the world with increased concerns about environmental protection and energy crisis. The depolymerization of cellulosic substrate to monomer is the rate-limiting step in the bioconversion of lignocellulose by cellulolytic microbes. Cellulosome, a multienzyme complex from anaerobic cellulolytic bacteria, can efficiently degrade the cellulosic substrates. Previous studies have shown that the reconstitution of cellulosome in vitro and its heterologous expression or display on the cell surface can help to solve the low yield problem of cellulosome in cellulolytic bacteria. This paper reviews the research progress in the reconstitution of cellulosome as well as its application in biorefinery, including the construction of cellulosome as well as different methods for cellulosome reconstitution and its surface display. This review will promote the understanding of cellulosome and its reconstitution.
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