Journal
BIOSENSORS & BIOELECTRONICS
Volume 140, Issue -, Pages 69-76Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111333
Keywords
Microfluidic biosensor; Fluorescence labeling; Smartphone video processing; Magnetic separation; Salmonella typhimurium
Categories
Funding
- National Natural Science Foundation of China [31802219]
- Walmart Foundation [SA1703161]
- Walmart Food Safety Collaboration Center
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Early screening of foodbome pathogens is a key to ensure food safety. In this study, we developed a microfluidic biosensor for online and sensitive detection of Salmonella based on immunomagnetic separation, fluorescence labeling and smartphone video processing. First, the immune magnetic nanoparticles were used to specifically separate and efficiently concentrate the target bacteria and the magnetic bacteria were formed. Then, the magnetic bacteria were labeled with the immune fluorescent microspheres and the fluorescent bacteria were formed. Finally, the fluorescent bacteria were continuously injected into the microfluidic chip on the smartphone-based fluorescent microscopic system, and the fluorescent spots were online counted using the smartphone App based on inter-frame difference algorithm to obtain the amount of the target bacteria. Under the optimal conditions, this proposed biosensor was able to quantitatively detect Salmonella typhimurium ranging from 1.4 x 10(2) to 1.4 x 10(6) CFU/mL, and its lower detection limit was 58 CFU/mL. This biosensor could be extended for detection of multiple foodbome pathogens using different fluorescent materials.
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