Journal
BIORESOURCE TECHNOLOGY
Volume 288, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.121526
Keywords
Feruloyl esterase; Lactobacillus crispatus; Secretory production; Escherichia coli; Ferulic acid
Funding
- Shandong Provincial Natural Science Foundation [ZR2016CB14]
- National Natural Science Foundation of China [31701576]
- China Postdoctoral Science Foundation [2017M622110]
- Shandong Postdoctoral Science Foundation [201703081]
- Key Research and Development Program of Shandong Province [2017CXGC0204, 2018YYSP002, 2018GSF121020]
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A feruloyl esterase producing strain was isolated and identified as Lactobacillus crispatus 5524. Its putative feruloyl esterase was heterogeneously expressed in Escherichia toll BL21 (DE3). Interestingly, the feruloyl esterase (FaeLcr) could be secreted into the culture medium with a relative high purity of 201.7 mg/L. FaeLcr was purified from the cell-free culture supernatant and appeared as a single protein band with the molecular mass of 28 kDa by SDS-PAGE. The optimal temperature and pH were determined as 65 degrees C and 7.0, and it showed prominent thermo-stability and alkali-stability. Furthermore, the purified FarLcr could release a maximal amount of 199 mu g ferulic acid from 0.2 g de-starched wheat bran. Meanwhile, when cultured this recombinant E. coli. strain in medium supplemented with 2 g de-starched wheat bran, 1.86 mg ferulic acid was also detected. These results suggested that the recombinant strain has a great potential application in feruloyl esterase and ferulic acid production.
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