Journal
BIORESOURCE TECHNOLOGY
Volume 285, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.121334
Keywords
Haematococcus pluvialis; gamma-Aminobutyric acid; Two-step cultivation; Fulvic acid; Salt supplementation
Funding
- National Natural Science Foundation of China [21766012]
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The primary goal of this study was to assess the roles of chemical factors and bioprocess strategies on a mixotrophic culture of the microalga Haematococcus pluvialis during gamma-aminobutyric acid (GABA) production. A two-stage strategy was used to increase the biomass and GABA accumulation of H. pluvialis. In stage I, mixotrophic growth of H. pluvialis in the presence of fulvic acid (FA) produced a high biomass (1.84 g L-1) with a GABA content of 25.45 mg g(-1). Furthermore, a maximum GABA content of 38.57 mg g(-1) was obtained when cells were cultured with 0.4 g L-1 NaCl under photoautotrophic conditions in stage II, whereas the carbohydrate content of cells sharply decreased from 26.68 to 18.22%. In addition, salt stress upregulated the expression of the gad and cam genes. The results of this study demonstrate an efficient strategy to produce GABA from the microalga H. pluvialis.
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