Journal
BIOORGANIC CHEMISTRY
Volume 89, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bioorg.2019.103040
Keywords
Neuromasts; Near Infrared (NIR) emission; Zebrafish; Styryl dye; Cyanine; Support cell; Excited state intramolecular proton transfer
Funding
- NIH [1R15GM126438-01A1]
- Coleman Endowment from The University of Akron
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Hearing loss is a significant public health problem, and the loss of sensory hair cells is one of two leading causes in humans. Advanced imaging reagents are desirable for understanding the role of the surrounding support cells in the loss or regeneration of the hair cells. A styryl dye was found to exhibit NIR emission (lambda(em) approximate to 684 nm) with a very large Stokes shift (Delta(v) approximate to 9190 cm(-1)) due to the incorporation of excited state intramolecular proton transfer (ESIPT) mechanism. When used to stain live zebrafish embryos, the probe was found to exhibit good selectivity in targeting neuromasts, which are sensory organs on the surface of the fish's body. The finding was verified by direct comparison with the known neuromast-labeling reagent, 4-Di-2-ASP. In contrast to the existing styryl dyes that label neuromast hair cells, the new probe labeled both neuromast hair cells and the surrounding support cells, while giving discernable signals. The study thus illustrated a useful tool to aid the developmental study of two closely related cell types on the mechanosensory sensory organ of zebrafish, which is a powerful animal model for hearing loss research.
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