Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 18, Pages 11582-11588Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b01687
Keywords
-
Categories
Funding
- Fonds Wetenschappelijk Onderzoek-Vlaanderen (FWO-Flanders) [G.0189.13N]
- BOF UAntwerp
Ask authors/readers for more resources
Human toxocariasis (HT) is a cosmopolitan zoonotic disease caused by the migration of the larval stage of the roundworm Toxocara canis. Current HT diagnostic methods do not discriminate between active and past infections. Here, we present a method to quantify Toxocara excretory/secretory antigen, aiming to identify active cases of HT. High specificity is achieved by employing nanobodies (Nbs), single domain antigen binding fragments from camelid heavy chain-only antibodies. High sensitivity is obtained by the design of an electrochemical magnetosensor with an amperometric read-out. Reliable detection of TES antigen at 10 and 30 pg/mL level was demonstrated in phosphate buffered saline and serum, respectively. Moreover, the assay showed no cross-reactivity with other nematode antigens. To our knowledge, this is the most sensitive method to quantify the TES antigen so far. It also has great potential to develop point of care diagnostic systems in other conditions where high sensitivity and specificity are required.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available