Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 18, Pages 11510-11513Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b03545
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Funding
- Fundamental Research Funds for the Central Universities
- National Sciences and Engineering Research Council of Canada
- Ontario Ministry of Research, Innovation and Science
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Herein, we described a novel plasmonic CRISPR Cas12a assay for the visual, colorimetric detection of grapevine viral infections. Our assay generates rapid and specific colorimetric signals for nucleic acid amplicons by combining the unique target-induced incriminate single-stranded DNase activity of Cas12a with plasmon coupling of DNA functionalized gold nanoparticles. The practical applicability of our plasmonic assay was successfully demonstrated through the detection of emerging red-blotch viral infections in grapevine samples collected from commercial vineyards.
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