Journal
ANALYTICA CHIMICA ACTA
Volume 1068, Issue -, Pages 104-110Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2019.04.018
Keywords
Fluorescence; Uranyl; DNAzyme; Entropy-driven amplification; Circular cleavage
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Funding
- Chongqing Key Laboratory of Catalysis and NewEnvironmental Materials [KFJJ2017033]
- Science and Technology Research Program of Chongqing Municipal Education Commission [KJ1706156]
- Open Project of State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials [17kffk06]
- Project of Wenzhou Science & Technology Bureau [W20170006]
- National Natural Science Foundation of China [31300819]
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A uranyl detection strategy with ultra-sensitivity was developed based on entropy-driven amplification and DNAzyme circular cleavage amplification. The cleavage of UO22+-specific DNAzyme produces a DNA fragment to initiate the entropy-driven amplification. Two DNA sequences released from the entropy-driven amplification are partly complementary. They can form an entire enzyme strand (E-DNA) of Mg2+-specific DNAzyme. The formed E-DNA can circularly cleave FAM-labeled probes on gold nanoparticles (AuNPs), causing the leaving of FAM from AuNPs and recovery of fluorescent signal. A linear relationship was obtained in the range from 30 pM to 5 nM between fluorescence intensity and concentration of UO22+. The limit of detection was low to 13 pM. This method showed a promising future for practical application in real water samples. (C) 2019 Elsevier B.V. All rights reserved.
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