Journal
ANALYTICA CHIMICA ACTA
Volume 1088, Issue -, Pages 137-143Publisher
ELSEVIER
DOI: 10.1016/j.aca.2019.08.059
Keywords
RNase H; Pyrene excimer; Inclusion ineraction; Fluorescence; Biosensor
Categories
Funding
- National Natural Science Foundation of China [21775035]
- Hunan Provincial Natural Science Foundation [2016JJ1005]
- Outstanding youth project of Hunan Provincial Department of Education [18B182]
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Here, we report a novel fluorescence method for the highly selective and sensitive detection of RNase H by combining the use of a dual-pyrene-labeled DNA/RNA duplex with supramolecular inclusion-enhanced fluorescence. Initially, the probe is in the off state due to the rigidness of the double-stranded duplex, which separates the two pyrene units. In the presence of RNase H, the RNA strand of the DNA/RNA duplex will be hydrolyzed, and the DNA strand transforms into a hairpin structure, bringing close the two pyrene units which in turn enter the hydrophobic cavity of a gamma-cyclodextrin. As a result, the pyrene excimer emission is greatly enhanced, thereby realizing the detection of RNase H activity. Under optimal conditions, RNase H detection can be achieved in the range from 0.08 to 4 U/mL, with a detection limit of 0.02 U/mL. (C) 2019 Elsevier B.V. All rights reserved.
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