Journal
ANALYTICA CHIMICA ACTA
Volume 1073, Issue -, Pages 30-38Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2019.04.054
Keywords
SiC; Immunoassay; Chronoamperometry; Chemiluminescence; Medical diagnosis
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Funding
- NanoConvergence Foundation - Ministry of Science, ICT and Future Planning (MSIP, Korea) [R201602210]
- Ministry of Trade, Industry and Energy (MOTIE, Korea)
- National Research Foundation of Korea [NRF-2017R1A2B4004077, NRF-2017R1A6A3A11034081]
- National Research Foundation of Korea [R201602210] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Highly N-doped SiC was presented as an optimal electrode for electrochemical immunoassays with a far higher sensitivity than chemiluminescence detection. As the first step, the electrochemical properties of highly N-doped SiC, such as the double-layer capacitance (C-dl), rate constant for electron transfer (k(app)) and ideal polarizable potential range (electrochemical window) were analyzed and compared with those of Au, Pt, and graphite electrodes. The highly N-doped SiC electrode was used for the quantification of oxidized 3,3',5,5'-tetramethylbenzidine (TMB) which was widely used as chromogenic substrate for commercialized immunoassay kits. In order to enhance the sensitivity for the quantification of the oxidized TMB the chronoamperometry was applied to avoid the background current of i-V measurement. Finally, the chronoamperometry based on the highly N-doped SiC electrode was applied to commercial immunoassay kits for the medical diagnosis of the human immunodeficiency virus (HIV) and the human hepatitis B surface antigen (hHBsAg). The chronoamperometric measurement based on the highly N-doped SiC electrode was proved to detect at far lower limits in comparison with the conventional optical density measurement as well as the chemiluminescence assay based on luminol as a chemiluminescent probe. (C) 2019 Elsevier B.V. All rights reserved.
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