Journal
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
Volume 61, Issue 2, Pages 150-161Publisher
AMER THORACIC SOC
DOI: 10.1165/rcmb.2019-0191ST
Keywords
flow cytometry; lung biology; reproducibility; cells
Funding
- National Institutes of Health [K08HL128867, U19AI135964, HL135124, AG049665, AI135964, R01ES027574, R01ES028829, K08HL105537, R35HL140039, R01HL130938]
- Department of Veterans Affairs [1IK2BX002401, I01 CX000911]
- National Institutes of Health/National Heart, Lung, and Blood Institute grant [U01HL137880]
- German Research Foundation [KFO309, 284237345, SFB1021, 197785619, EXC2026, 390649896]
- Department of Defense [PR141319]
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Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.
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