Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 189, Issue 11, Pages 2171-2180Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2019.07.013
Keywords
-
Categories
Funding
- Agence Nationale de la Recherche [ANR-13-JSV1-0010-01, ANR-12-BS08-0022]
- Academie Nationale de Medecine Nestle-Waters award
- Convergence Universite Pierre et Marie Curie grant
- Cardiovasculaire Rein Obesite Diabete Domaine d'Interet Majeur-Region Be de France [2013-COD130042 CVG1205]
- Agence Nationale de la Recherche (ANR) [ANR-13-JSV1-0010] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
Most kidney stones are made of calcium oxalate crystals. Randall's plaque, an apatite deposit at the tip of the renal papilla, is considered to at the origin of these stones. Hypercalciuria may promote Randall's plaque formation and growth. We analyzed whether long-term exposure of Abcc6(-/-) mice (a murine model of Randall's plaque) to vitamin D supplementation, with or without a calcium-rich diet, would accelerate the formation of Randall's plaque. Eight groups of mice (including Abcc6(-/-) and wild type) received vitamin D alone (100,000 UI/kg every 2 weeks), a calcium-enriched diet alone (calcium gluconate 2 g/L in drinking water), both vitamin D supplementation and a calcium-rich diet, or a standard diet (controls) for 6 months. Kidney calcifications were assessed by 3-dimensional microcomputed tomography, mu-Fourier transform infrared spectroscopy, field emission-scanning electron microscopy, transmission electron microscopy, and Yasue staining. At 6 months, Abcc6(-/-) mice exposed to vitamin D and calcium supplementation developed massive Randall's plaque when compared with control Abcc6(-/-) mice (P < 0.01). Wild-type animals did not develop significant calcifications when exposed to vitamin D. Combined administration of vitamin D and calcium significantly accelerates Randall's plaque formation in a murine model. This original model raises concerns about the cumulative risk of vitamin D supplementation and calcium intakes in Randall's plaque formation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available