Journal
AGING-US
Volume 11, Issue 17, Pages 7294-7306Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/aging.102266
Keywords
apoptosis; inflammation; autophagy; NF-kappa B pathway; nucleus pulposus cells
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Funding
- Natural Science Foundation of China [81572202]
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The NF-kappa B pathway has been reported to play a very important role in the process of intervertebral disc degeneration (IVDD). Our results demonstrated that knockdown of NF-kappa B with P65-siRNA can significantly decrease cell apoptosis and the expression of pro-inflammation factors TNF-alpha and IL-1 beta in LPS-induced nucleus pulposus cells (NPCs). However, the molecular mechanism of NF-kappa B pathway exerting anti-inflammation and anti-apoptosis function remains unclear. Some researchers reported that inhibiting NF-kappa B pathway can attenuate the catabolic effect by promoting autophagy during inflammatory conditions in rat nucleus pulposus cells. Therefore, we hypothesized that in human NPCs, inhibiting NF-kappa B pathway may also promote autophagy. Our results indicated that after knockdown of NF-kappa B, the autophagy was significantly increased and the expression of p-AKT and p-mTOR protein markedly decreased, but the level of autophagy was inhibited after treatment with AKT activator SC79, suggesting the involvement of AKT/mTOR-mediated autophagy was under autophagy activation. However, both LPS-induced NPCs apoptosis and expression of pro-inflammation factors were further increased by pretreatment with the autophagy inhibitor chloroquine (CO). These suggested that inhibiting NF-kappa B pathway can promote autophagy and decrease apoptosis and inflammation response in LPS-induced NPCs. Meanwhile, autophagy triggered by NF-kappa B inhibition plays a protective role against apoptosis and inflammation.
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