Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 35, Pages 31729-31734Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b11599
Keywords
immunoglobulin G; gold nanocluster; fluorescence; dot-blot immunoassay; antigen-antibody interaction
Funding
- National Natural Science Foundation of China [21675024, 21804021]
- Program for Innovative Leading Talents in Fujian Province [2016B016]
- Joint Funds for the Innovation of Science and Technology, Fujian Province [2016Y9056]
- Natural Science Foundation of Fujian Province [2017J01575]
- Startup Fund for Scientific Research, Fujian Medical University [2017XQ1014]
- Science and Technology Project of Quanzhou [2018Z090]
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Few-atom gold nanoclusters (AuNCs) have been fabricated and used for various fields owing to their remarkable optical and photophysical features. However, the rational design for the antibody-mediated synthesis of fluorescent AuNCs for direct antigen-antibody reactions remains unexplored. In this work, immunoglobulin G (IgG)-functionalized AuNCs (IgG-AuNCs) were successfully prepared via a facile and fast biomineralization process. The generated IgG-AuNCs can emit intense red fluorescence with a high photoluminescence quantum yield. Besides strong emission, the bioactivity of IgG on the IgG-AuNCs can be retained. Surface plasmon resonance measurements suggested that IgG-AuNCs can bind to goat anti-human IgG with an affinity constant of 6.21 X 10(-8) M. A simple detection method was then developed using a dot-blot immunoassay with IgG-AuNCs as fluorescent tags. Experimental results confirmed that the IgG-AuNC-based fluorescent reporters had many advantages such as low nonspecific adsorption and good photostability, offering immense potential for the development of efficient biosensors. This work can be extended to other specific antibodies to produce multifunctional AuNCs and utilized to detect and monitor targeted analytes and biological events of interest.
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