Journal
PLANTS-BASEL
Volume 8, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/plants8080257
Keywords
quinoa; quinoa protein concentrates; antioxidant activity; hydrolysates; zebrafish embryos
Categories
Funding
- Universidad Tecnica de Ambato (Ecuador) [CPU-1373-2014]
Ask authors/readers for more resources
Red, black and white seeds quinoa were germinated at 28 degrees C during 24 (G1), 48 and 72 h (G3). Red quinoa presented a higher percentage of germination with a value of 46% of germination at 72 h. Quinoa protein isolate (QPI) was obtained by alkaline extraction (pH 8.0) followed by an isoelectric precipitation (pH 4.5) from white, red and black quinoa seeds, germinated QPI-G1 or QPI-G3 and non-germinated QPI-NG, Chenopodium quinoa Willd var. Real. QPI-G1, QPI-G3 and QPI-NG were subject to a simulated gastric digestion (DG) and in vitro duodenal digestion (DD). The antioxidant activity was evaluated using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH), azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) methods. Gastric and duodenal digest of QPI-NG and QPI-G1 and QPI-G3 from white, red and black quinoa presented antioxidant activity. QPI-G1-DD of white quinoa presented the highest antioxidant activity with a DPPH value of 167.98 mu moL TE/g of digest, QPI-G1-DD of red quinoa with an ABTS value of 204.86 mu moL TE/g of digest and QPI-G1-DD of black quinoa with an ORAC value of 401.42 mu moL TE/g of digest. QPI-G3-DD of white quinoa presented higher antioxidant activity with a DPPH value of 186.28 mu moL TE/g of sample, QPI-G3-DD of red quinoa with an ABTS value of 144.06 mu moL TE/g of digest and QPI-G3-DD of black quinoa with an ORAC value of 395.14 mu moL TE/g of digest. The inhibition of reactive oxygen species (ROS) production in the zebrafish embryo model (Danio rerio) was evaluated. Protein profiles of QPI from white, red and black from germinated quinoa and non-germinated quinoa were similar with proteins between 10 kDa to 100 kDa with the presence of globulins 11S and 7S and 2S albumins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available