Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2019.00174
Keywords
hydrolyzed keratin; gelatin; GPTMS; TFE; electrospinning; tissue engineering
Funding
- BIOMEMBRANE project (M-ERA.NET2 project) [4246]
- Kerapack project [MANUNET MNET17/NMAT-0060]
- University of Pisa [PRA_2018_68]
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The aim of this study is the analysis and characterization of a hydrolyzed keratin-based biomaterial and its processing using electrospinning technology to develop in vitro tissue models. This biomaterial, extracted from poultry feathers, was mixed with type A porcine gelatin and cross-linked with gamma-glycidyloxy-propyl-trimethoxy-silane (GPTMS) to be casted initially in the form of film and characterized in terms of swelling, contact angle, mechanical properties, and surface charge density. After these chemical-physical characterizations, electrospun nanofibers structures were manufactured and their mechanical properties were evaluated. Finally, cell response was analyzed by testing the efficacy of keratin-based structures in sustaining cell vitality and proliferation over 4 days of human epithelial, rat neuronal and human primary skin fibroblast cells.
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