Journal
ARCHIVES OF VIROLOGY
Volume 162, Issue 2, Pages 543-548Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-016-3129-8
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Funding
- Ministry of Education, Culture, Science, Sports and Technology (MEXT) of Japan [16H06429, 16H06431, 16K21723]
- Ministry of Education, Culture, Sports, Science and Technology
- Ministry of Health, Labour and Welfare, Japan (MEXT)/JSPS KAKENHI [16H05805]
- Japan Initiative for Global Research Network on Infectious Diseases (J-GRID), MEXT Japan
- Grants-in-Aid for Scientific Research [16H02863, 16H06431, 16H05805] Funding Source: KAKEN
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To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4(+) T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed < 80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.
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