Journal
FRONTIERS IN PEDIATRICS
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fped.2019.00283
Keywords
acute fulminant myocarditis; long non-coding RNAs; microarray; messenger RNA; functional analysis
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Funding
- Science and Technology Development Plan of Jinan City [201805020]
- Taishan scholars [ts201511099]
- National Natural Science Foundation of China [8187020860]
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Long non-coding RNA (IncRNA) has been associated with human diseases. To study the role of IncRNA in the pathogenic mechanism of acute fulminant myocarditis (AFM), we used a microarray to analyze IncRNA and messenger RNA (mRNA) expression in leukocyte samples from AFM patients and normal children. In total, using a 2/0.5-fold change and P < 0.05 as the cutoff criteria, we found that 3,101 IncRNAs and 2,170 mRNAs were differentially expressed in AFM patients. Quantitative real-time polymerase chain reaction (RT-qPCR) analysis was used to verify the microarray data. Eight differentially expressed molecules were randomly selected, including 3 upregulated IncRNAs, 3 downregulated IncRNAs, and 2 upregulated mRNAs. Among them, 7 expression profiles were consistent with the microarray results. Gene Ontology enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis were used to investigate the biological functions of these genes. Establishment of a IncRNA-mRNA co-expression network and IncRNA target predication were performed to study the molecular interactions of these molecules. Our study is the first to use microarrays to examine the IncRNA and mRNA expression profiles associated with AFM, and the results indicate that the immune system plays an important role in AFM. These findings may provide a new perspective for the pathogenesis, diagnosis, and therapy of AFM.
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