Journal
JOURNAL OF SYNCHROTRON RADIATION
Volume 26, Issue -, Pages 1388-1399Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600577519003576
Keywords
footprinting; structural dynamics; beamline; solution state
Categories
Funding
- Sydor Instruments [DE-SC0007482]
- National Science Foundation, Division of Biological Infrastructure [1228549, 1255340]
- DOE Office of Science [DE-SC0012704]
- Center for Synchrotron Biosciences from the National Institute of Biomedical Imaging and Bioengineering [P30-EB-00998]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1255340, 1228549] Funding Source: National Science Foundation
- U.S. Department of Energy (DOE) [DE-SC0007482] Funding Source: U.S. Department of Energy (DOE)
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Hydroxyl-radical mediated synchrotron X-ray footprinting (XF) is a powerful solution-state technique in structural biology for the study of macromolecular structure and dynamics of proteins and nucleic acids, with several synchrotron resources available to serve the XF community worldwide. The XFP (Biological X-ray Footprinting) beamline at the NSLS-II was constructed on a three-pole wiggler source at 17-BM to serve as the premier beamline for performing this technique, providing an unparalleled combination of high flux density broadband beam, flexibility in beam morphology, and sample handling capabilities specifically designed for XF experiments. The details of beamline design, beam measurements, and science commissioning results for a standard protein using the two distinct XFP endstations are presented here. XFP took first light in 2016 and is now available for general user operations through peer-reviewed proposals. Currently, beam sizes from 450 mu m x 120 mu m to 2.7mm x 2.7mm (FWHM) are available, with a flux of 1.6 x 10(16)photonss(-1) (measured at 325mA ring current) in a broadband (similar to 5-16keV) beam. This flux is expected to rise to 2.5 x 10(16)photonss(-1) at the full NSLS-II design current of 500mA, providing an incident power density of >500Wmm(-2) at full focus.
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