Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 149, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/59920
Keywords
Immunology and Infection; Issue 149; Antivirals; drug development; entry inhibitors; viral entry; binding analysis; molecular docking; Autodock; PyMol; UCSF Chimera
Categories
Funding
- Ministry of Science and Technology of Taiwan [MOST107-2320-B-037-002, MOST106-2320-B-038-021, MOST107-2320-B-038-034-MY3]
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Antiviral assays that mechanistically examine viral entry are pertinent to discern at which step the evaluated agents are most effective, and allow for the identification of candidate viral entry inhibitors. Here, we present the experimental approaches for the identification of small molecules capable of blocking infection by the non-enveloped coxsackievirus A16 (CVA16) through targeting the virus particles or specific steps in early viral entry. Assays include the time-of-drug-addition analysis, flow cytometry-based viral binding assay, and viral inactivation assay. We also present a molecular docking protocol utilizing virus capsid proteins to predict potential residues targeted by the antiviral compounds. These assays should help in the identification of candidate antiviral agents that act on viral entry. Future directions can explore these possible inhibitors for further drug development.
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