Journal
GENES
Volume 10, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/genes10070483
Keywords
sea bream; development; larvae; symbionts; bacteria
Categories
Funding
- European Union's Horizon 2020 research and innovation programme [818290]
- H2020 Societal Challenges Programme [818290] Funding Source: H2020 Societal Challenges Programme
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One of the most widely reared fish in the Mediterranean Sea is Sparus aurata. The succession of S. aurata whole-body microbiota in fertilized eggs, five, 15, 21 and 71 days post hatch (dph) larvae and the contribution of the rearing water and the provided feed (rotifers, Artemia sp. and commercial diet) to the host's microbiota was investigated by 454 pyrosequencing of the 16S rRNA gene diversity. In total, 1917 bacterial operational taxonomic units (OTUs) were found in all samples. On average, between 93 +/- 2.1 and 366 +/- 9.2 bacterial OTUs per sample were found, with most of them belonging to Proteobacteria and Bacteroidetes. Ten OTUs were shared between all S. aurata stages and were also detected in the rearing water or diet. The highest OTU richness occurred at the egg stage and the lowest at the yolk sac stage (5 dph). The rearing water and diet microbial communities contributed in S. aurata microbiota without overlaps in their microbial composition and structure. The commercial diet showed higher contribution to the S. aurata microbiota than the rearing water. After stage D71 the observed microbiota showed similarities with that of adult S. aurata as indicated by the increased number of OTUs associated with gamma-Proteobacteria and Firmicutes.
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