Journal
GENES
Volume 10, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/genes10070486
Keywords
Steller sea lion; Eumetopias jubatus; genome; microfluidic partitioning; nanopore; marine animal
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Funding
- CanSeq150 program of Canada's Genomics Enterprise
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The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.
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