Journal
CATALYSTS
Volume 9, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/catal9060526
Keywords
biocatalysis; extremophile; 5-hydroxymethylfurfural; 5-hydroxymethylfuroic acid; platform chemicals; whole cells
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Funding
- National Natural Science Foundation of China [21646014, 21776134, 21776136]
- program of Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture [XTE1851]
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Value-added utilization of biomass-derived 5-hydroxymethylfurfural (HMF) to produce useful derivatives is of great interest. In this work, extremely radiation resistant Deinococcus wulumuqiensis R12 was explored for the first time as a new robust biocatalyst for selective oxidation of HMF to 5-hydroxymethylfuroic acid (HMFCA). Its resting cells exhibited excellent catalytic performance in a broad range of pH and temperature values, and extremely high tolerance to HMF and the HMFCA product. An excellent yield of HMFCA (up to 90%) was achieved when the substrate concentration was set to 300 mM under the optimized reaction conditions. In addition, 511 mM of product was obtained within 20 h by employing a fed-batch strategy, affording a productivity of 44 g/L per day. Of significant synthetic interest was the finding that the D. wulumuqiensis R12 cells were able to catalyze the selective oxidation of other structurally diverse aldehydes to their corresponding acids with good yield and high selectivity, indicating broad substrate scope and potential widespread applications in biotechnology and organic chemistry.
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